While Barcelona meet Arsenal over two legs on 30/31 March and 6/7 April, Olympique Lyonnais play French rivals FC Girondins de Bordeaux, FC Bayern München take on Manchester United FC in a replay of the 1999 Camp Nou final and FC Internazionale Milano meet PFC CSKA Moskva.
In the semi-finals, Barcelona or Arsenal will take on Inter or CSKA while the remaining French representatives face Bayern or United.
The final will be played at Madrid's Santiago Bernabéu stadium, the first in its new Saturday slot of 22 May.
2009/10 UEFA Champions League drawQuarter-finals : 30/31 March & 6/7 April
1: Olympique Lyonnais (FRA) v FC Girondins de Bordeaux (FRA)
2: FC Bayern München (GER) v Manchester United FC (ENG)
3: Arsenal FC (ENG) v FC Barcelona (ESP, holders)
4: FC Internazionale Milano (ITA) v PFC CSKA Moskva (RUS)