Sevilla striker Alvaro Negredo won the battle to replace record scorer David Villa, who failed to recover from a broken tibia in time, while Adrian Lopez was overlooked despite scoring on his debut in the 2-0 win over Serbia on Saturday.
Manchester United goalkeeper David de Gea misses out and a knee injury meant Barcelona defender Carlos Puyol was already ruled out.
Spain won all eight of their qualifiers for Euro 2012 and are in Group C for the finals along with Italy, against whom they play their opening game on 10 June, Republic of Ireland and Croatia.
Spain Euro 2012 Squad :Goalkeepers: Iker Casillas (Real Madrid), Pepe Reina, (Liverpool), Victor Valdes (Barcelona)
Defenders: Alvaro Arbeloa (Real Madrid), Sergio Ramos (Real Madrid), Juanfran (Atletico Madrid), Jordi Alba (Valencia), Raul Albiol (Real Madrid), Gerard Pique (Barcelona), Javi Martinez (Athletic Bilbao)
Midfielders: Xavi (Barcelona), Sergio Busquets (Barcelona), Andres Iniesta (Barcelona), Xabi Alonso (Real Madrid), David Silva (Manchester City), Santi Cazorla (Malaga), Cesc Fabregas (Barcelona), Juan Mata (Chelsea)
Forwards: Jesus Navas (Sevilla), Pedro (Barcelona), Fernando Llorente (Athletic Bilbao), Fernando Torres (Chelsea), Alvaro Negredo (Sevilla)