Scottish Premier League champions Rangers have been drawn against Portuguese side Sporting Lisbon.
Manchester City, who are third in the Premier League, will travel to Greece to play Aris Salonika for the first leg. Aris beat Atletico Madrid in the Spanish capital with a 3-2 away win, a result which knocked the holders out.
Other tasty ties include Seville against Porto, who between them won three of the five Uefa Cup finals between 2003 and 2007, while Ajax take on Anderlecht of Belgium.
All first legs will be played on 7 February with the return games taking place a week later, while the last 16 is scheduled for March 10 and 17.
2011 Europa League draw (last 32) :===================================
Napoli v Villarreal
Rangers v Sporting
Sparta Prague v Liverpool
Anderlecht v Ajax
Lech Poznan v Braga
Besiktas v Dynamo Kiev
Basle v Spartak Moscow
Young Boys v Zenit St Petersburg
Aris Salonika v Man City
PAOK Salonika v CSKA Moscow
Sevilla v FC Porto
Rubin Kazan v FC Twente
Lille v PSV
Benfica v VfB Stuttgart
Bate Borisov v PSG
FC Metalist Kharkiv v Bayer Leverkusen